human cerebral microvascular endothelial cells (Cedarlane)

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Cedarlane human cerebral microvascular endothelial cells
Human Cerebral Microvascular Endothelial Cells, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cerebral+microvascular+endothelial+cells/Immortalized+Human+Cerebral+Microvascular+Endothelial+Cell+Line+(hCMEC%2FD3)/pm41934661-289-0-6
Average 95 stars, based on 97 article reviews

human cerebral microvascular endothelial cells - by Bioz Stars, 2026-09

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Article Title: Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols.
Article Snippet: Cryopreservation is the process of preserving biological matter at subzero temperatures for long-term storage.. During cryopreservation, cells are susceptible to various injuries that can be mitigated by controlling the cooling and warming profiles and cryoprotective agent (CPA) addition and removal procedures.. Mathematical modeling of the changing cell volume at different temperatures can greatly reduce the experiments needed to optimize cryopreservation protocols.

Article Title: In‐vitro Assessment of BCRP‐Mediated Efflux of Antiseizure Medications in Human Blood‐Brain Barrier Cell Model
Article Snippet: Human cerebral microvascular endothelial cells (hCMEC/D3; cat. no. CLU‐512) were bought from Cedarlane Laboratories (Burlington, Canada).

Agarose Gel Electrophoresis:

Article Title: Methods and compositions for delivery of immunotherapy agents across the blood-brain barrier to treat brain cancer
Article Snippet: .. Primary human astrocytes (Lonza Bioscience), human brain microvascular pericytes (HBVP, ScienCell Research Laboratories) and human cerebral microvascular endothelial cells (hCMEC/D3; Cedarlane) were then seeded onto the agarose gel in a 1:1:1 ratio (1500 cells of each type). ..

Cell Culture:

Article Title: Tuneable Permeability of Cellulose Nanofibrils-based Membranes in Next-Generation Barrier-On-Chip Systems.
Article Snippet: .. Human cerebral microvascular endothelial cells (HCMEC/D3) (Cederlane Laboratories, Canada, Catalog No. CLU512, RRID: CVCL_U985) were cultured in Ham’s F12 (Sigma–Aldrich/ Merk) supplemented with 5% fetal bovin serum (Sigma–Aldrich/ Merk), 5% L-glutamine (Sigma–Aldrich/Merk), 0.25% penicillin (10,000 IU/mL), streptomycin (10,000 μg/mL) (Sigma–Aldrich/ Merk), 1 μg/mL hydrocortisone 21-hemisuccinate sodium salt (Sigma–Aldrich/Merk), 50 μg/mL L-ascorbic acid (Sigma–Aldrich/ Merk), 0.75 U/mL heparin (Sigma–Aldrich/Merk), and 1 ng/mL human basic fibroblast growth factor (Sigma–Aldrich/Merk). ..

Article Title: Plasma membrane impacts of particulate matter on the blood-brain barrier.
Article Snippet: .. Human cerebral microvascular endothelial cells (hCMEC/D3) were purchased from Cedarlane. hCMEC/D3 cells was cultured in EBM-2 endothelial basal medium (Lonza, #190860), supplemented with 5% fetal bovine serum (Gibco, #12483020), 1% chemically defined lipid concentrate (Life technologies, #11905031), 5 μg/mL ascorbic acid (Sigma, #A4544), 1 ng/mL bFGF (human basic fibroblast growth factor, Sigma, #F0291), 10 mM HEPES (Life Technologies, #15630-080), 1.4 μM hydrocortisone (Sigma, #H0135) and 1% penicillin, 10000 units streptomycin, 10000 μg/mL (Life technologies, #15140-122). .. Inserts and flasks/Petri dishes were covered with a thin collagen I (Cultrex® Rat Collagen I lower viscosity, R&D Systems, Trevigen, #3443-100-01) coating at 150 μg/mL at 37 ◦C for at least 1 h. The cells were cultured in an incubator at 37 ◦C with 5% CO2 and passaged twice a week using trypsin-EDTA 0.05% (v/v) until at least 95% confluency was reached.

Article Title: Tuneable Permeability of Cellulose Nanofibrils‐based Membranes in Next‐Generation Barrier‐On‐Chip Systems
Article Snippet: .. Human cerebral microvascular endothelial cells (HCMEC/D3) (Cederlane Laboratories, Canada, Catalog No. CLU512, RRID:CVCL_U985) were cultured in Ham's F12 (Sigma–Aldrich/Merk) supplemented with 5% fetal bovin serum (Sigma–Aldrich/Merk), 5% L‐glutamine (Sigma–Aldrich/Merk), 0.25% penicillin (10,000 IU/mL), streptomycin (10,000 μg/mL) (Sigma–Aldrich/Merk), 1 μg/mL hydrocortisone 21‐hemisuccinate sodium salt (Sigma–Aldrich/Merk), 50 μg/mL L‐ascorbic acid (Sigma–Aldrich/Merk), 0.75 U/mL heparin (Sigma–Aldrich/Merk), and 1 ng/mL human basic fibroblast growth factor (Sigma–Aldrich/Merk). ..

Article Title: PDMS Membranes Drilled by Proton Microbeam Writing: A Customizable Platform for the Investigation of Endothelial Cell–Substrate Interactions in Transwell-like Devices
Article Snippet: .. Human Cerebral Microvascular Endothelial Cells (HCMEC/D3, Cederlane, Burlington, NC, USA) were cultured in EndoGRO MV culture media (Millipore, Burlington, MA, USA) and used between passages 27 and 30. ..

Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
Article Snippet: For expansion, basic fibroblast growth factor (30 ng/ml, PeproTech, Rocky Hill, NJ, USA) and leukemia inhibitory factor (10 ng/ml, Merck Millipore, Darmstadt, Germany) were added to the culture medium. .. Human cerebral microvascular endothelial cells (hCMEC/D3; Cedarlane, Ontario, Canada) were cultured in an endothelial growth medium (EGMTM-2 SingleQuotsTM; Lonza, Verviers, Belgium) in a 150 μg/ml Col-coated flask. .. Human brain vascular pericytes (HBVP; ScienCell Research Laboratories, Carlsbad, CA, USA) were cultured in a pericyte medium (ScienCell Research Laboratories) containing 2% FBS, 1% penicillin-streptomycin and a PC growth supplement (ScienCell Research Laboratories).

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